isolation of nocardia from patients with bronchopulmonary disorders by using paraffin bait technique
نویسندگان
چکیده
170 patients admitted to shariaty hospital pulmonary ward were studied for pulmonary nocardiosis during 17 months (aug 1990-jan 1992). paraffin baiting was used and nocardia asteroides isolated in five cases from sputum, pleural fluid and bronchial washing. the technique used in this study inhibits growth of other organism and yeast and is reliable, simple and cheap for isolation of nocardia from clinical specimens especially in immuno deficient patients.
منابع مشابه
Application of paraffin bait technique to the isolation of Nocardia asteroides from clinical specimens.
The principal findings of a study for further evaluating paraffin baiting as a routine laboratory diagnostic procedure in the isolation of Nocardia asteroides, the etiological agent of nocardiosis, are reported.
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Abstract Background and Objective: The Isolation of Nocardia species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for Nocardia species. Material and Met...
متن کاملComparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum.
Of 1,510 sputum samples examined from 1,016 patients with bronchopulmonary disorders, Nocardia asteroides was isolated from 67 samples by paraffin baiting, as compared with only 30 isolations by the conventional technique of culturing on Sabouraud dextrose agar. This higher efficacy of the paraffin bait technique was found to be statistically significant (P less than 0.001).
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Background & Aims: Nocardia are gram-positive, aerobic, relative acid-fast, and opportunistic bacteria, and one of the causes of systematic infection around the world. The natural habitats of these bacteria are soil and dust. Hospitalized patients with immune deficiency are at high risk of transmission of opportunistic infection. Due to these facts and...
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عنوان ژورنال:
iranian journal of public healthجلد ۲۲، شماره ۱-۴، صفحات ۵۳-۶۲
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